High-Performance Liquid-Chromatographic Determination Of Diclofenac Diethylammonium In Gels

A simple and precise liquid chromatographic method was developed for the estimation of Diclofenac Diethylammonium from gels. The drug was chromatographed on a reverse phase C18 column. The eluants were monitored at a wavelength of 282 nm utilizing a mixture of 0.01 M disodium hydrogen orthphosphate and acetonitrile (50 : 50).The method was statistically validated for its accuracy, precision, reproducibility. The linearity was found to be in the range of 0 - 500 mcg/ml.Due to its simplicity and accuracy the authors were of the firm opinion that the method can be used for routine Quality Control analysis. The method do not require any specific sample preparation except for use of a gaurd column before the analytical column and a suitable prefilter attached to syringe prior to injection.