Clinical significance of CD45RO expression on peripheral blood mononuclear cells in HTLV-I-infected individuals.

The phenotype of peripheral blood mononuclear cells (PBMC) was examined in 13 healthy volunteers, 26 HTLV-I carriers, and 58 ATL patients (22 smouldering, five chronic, 24 acute, and seven lymphoma type). The percentage of CD4(+), CD25(+), CD28(+) and CD45RO(+) cells in the PBMC of the chronic and acute type patients was significantly higher than that of the volunteers, whereas the percentage of CD8(+) and CD45RA(+) cells in these patients was significantly low. The histogram for CD45RO fluorescence intensity (FI) revealed two patterns: pattern A consisted of CD45RO(+) cells with high FI (CD45RO(high)) and intermediate FI (CD45RO(int)). Pattern B consisted exclusively of CD45RO(high). Pattern A was evident in all volunteers. The percentage of subjects showing pattern B was increased in an order that reflected disease progression. In the patients with pattern A, the CD45RO(int) cells were CD4(+) and CD8(-), and the FI of CD2, CD3, and Fas within the CD45RO(int) cells appeared to be lower than that within the CD45RO(high) cells. The acute type patients with pattern A had a significantly longer survival curve than that of these patients with pattern B. These results suggest that the presence of CD45RO(int) cells may be related to protection against disease progression in HTLV-I-infected individuals.