Oxatomide attenuates the priming capacity on polymorphonuclear leukocytes of nasal lavage fluid obtained after allergen challenge

The priming activity on polymorphonuclear leukocytes (PMLs) of nasal lavage fluid obtained from children with seasonal allergic rhinitis after specific allergic challenge, before and after a 14-day treatment period with orally administered oxatomide 12.5 mg twice daily, was investigated by means of chemiluminescence. Luminol-amplified chemiluminescence of PMLs activated with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA) was evaluated in the presence or absence of nasal lavage fluid, A significant increase in the chemiluminescence response of fMLP-stimulated PMLs was induced by nasal lavage fluid. No significant change was observed in PMA-stimulated PMLs. Oxatomide treatment significantly reduced the effect of nasal lavage fluid on PMLs, These data suggest a priming potency on PMLs of nasal lavage fluid obtained from patients with seasonal allergic rhinitis after specific challenge, probably because of the presence of various inflammatory mediators, Oxatomide treatment significantly reduced this priming capacity.This effect may be attributed to the inhibition of cellular recruitment or to the reduction of mediator release at the inflammation site.