A glucose biosensor based on electrodeposition of palladium nanoparticles and glucose oxidase onto Nafion-solubilized carbon nanotube electrode

Biosensors and Bioelectronics, no. 11 (2005): 2341-2346

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摘要

Electrodeposition was used for the co-deposition of glucose oxidase (GOx) enzymes and palladium nanoparticles onto a Nafion-solubilized carbon nanotube (CNT) film. The co-deposited Pd–GOx–Nafion CNT bioelectrode retains its biocatalytic activity and offers an efficient oxidation and reduction of the enzymatically liberated H2O2, allowing ...更多

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简介
  • The incorporation of enzymes with a transducer allows one to fabricate highly sensitive and selective enzyme-based biosensors.
  • Of particular interest is the use of carbon nanotube as an electrochemical transducer
  • It possesses excellent electron transfer rate (Nugent et al., 2001), which is much better than conventional carbon electrodes, and allows surface chemistry for tethering foreign biomaterials such as enzymes and nucleic acids (Williams et al, 2002)
重点内容
  • The incorporation of enzymes with a transducer allows one to fabricate highly sensitive and selective enzyme-based biosensors
  • The formation of Pd nanoparticles on Nafion-carbon nanotube (CNT) film indicates that the CNT sidewalls are not entirely masked by the Nafion and there are accessible areas for the co-deposition of Pd–glucose oxidase (GOx) biocomposites
  • It has been suggested that the solubilization action of Nafion is very similar to other polymeric materials bearing polar side chains, which ‘wrap’ around the CNTs, and the electrocatalytic properties of CNTs are not impaired by the Nafion under optimal conditions (Wang et al, 2003)
  • Dispersing CNTs in 0.5% Nafion ensures that the amount of CNTs are controllably cast onto glassy carbon electrode (GCE) and the CNTs still retain its electrocatalytic carbon surface
  • The sidewalls of the CNTs are not completely masked by the 0.5% Nafion and there are accessible site for the incorporation of Pd–GOx biocomposites
  • The relative deviation for 10 times continuous determinations of 5 mM glucose is less than ∼3%, which indicates that the optimized Pd(1 mM)–GOx–Nafion CNT bioelectrode has a good operational stability
结果
  • Electron micrographs of Pd–GOx biocomposites on CNTs. From the TEM images (Fig. 1), roughly spherical Pd nanoparticles (∼2–6 nm) are randomly decorated on the walls of the various CNTs. It is interesting to note that Pd nanoparticles are formed instead of a conformal Pd coating.
  • The formation of Pd nanoparticles on Nafion-CNT film indicates that the CNT sidewalls are not entirely masked by the Nafion and there are accessible areas for the co-deposition of Pd–GOx biocomposites.
  • Dispersing CNTs in 0.5% Nafion ensures that the amount of CNTs are controllably cast onto GCE and the CNTs still retain its electrocatalytic carbon surface
结论
  • A more controllable, stable and reproducible deposition of CNTs film onto GCE can be achieved using homogeneous solution of (0.5%)Nafion-CNT.
  • The optimized Pd–GOx–Nafion CNT bioelectrodes has a good glucose-biosensing capability.
  • The relative deviation for 10 times continuous determinations of 5 mM glucose is less than ∼3%, which indicates that the optimized Pd(1 mM)–GOx–Nafion CNT bioelectrode has a good operational stability.
  • The optimized Pd(1 mM)–GOx–Nafion CNT bioelectrode lost at most ∼5% of the initial response after a storage period of 14 days.
  • After 50 days of storage, the Pd(1 mM)–GOx–Nafion bioelectrode gradually began to lose its initial response and drop to ∼40%.
  • When a higher concentration of PdCl2 (1.5 mM) is used for the co-deposition of GOx onto Nafion-CNT film, the
基金
  • As-fabricated Pd(1.5 mM)–GOx–Nafion CNT bioelectrodes lost more than 5% of its initial response after a storage period of about 6 days
引用论文
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