Effect Of Opu Interval And Bst Treatment On Embryo Production In Buffalo

The objective of present study was to evaluate the effect of OPU interval and the effect of bST treatment on embryo production in buffalo. Sixteen females were assigned in 4 groups, in a 2x2 experimental design, with main effects of bST (0 or 500mg) and interval of OPU session (every 7 or 14 days), as follows: G-CONT7: OPU once a week; G-CONT14: OPU each 14 days; G-bST7: OPU once a week + 500mg of bST and G-bST14: OPU each 14 days + 500mg of bST. Animals of G-CONT7 and G-bST7 were submitted to 8 OPU sessions, and those of G-CONT14 and G-bST14 to 4 OPU sessions. Overall, OPU once a week (without bST) reduced the number of aspirated follicles, and the total and viable oocytes. Despite of this, OPU every 14 days + bST increased the number of degenerated oocytes, and reduced the number of blastocysts produced on days 6 and 7.