A monoclonal antibody which can distinguish between the two isotypes of human C4
Molecular Immunology(1987)
摘要
A monoclonal antibody to Human C4 (L003) has been shown to bind to the polymorphic C4d fragment of the α-chain of C4. Another monoclonal antibody (L001) was shown to bind to the β-chain. L003 reacts differently with the two isotypes of C4, C4-A and C4-B. The equilibrium constant of L003 for C4-B is approx. 7-fold higher than that for C4-A, while L001 has similar affinity for both C4 types. Also, L003 binding to C4-A is very much more pH dependent than is its binding to C4-B. These observations explain the very useful property of L003 in being able to separate the two isotypes by affinity chromatography.
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关键词
BSA,DAB,EDTA,NCS,SDS,TBS
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