Branched chain α-keto acid dehydrogenase in tissues from rainbow trout (Salmo gairdnerii)
Comparative biochemistry and physiology. B. Comparative biochemistry(1984)
摘要
Mitochondria isolated from skeletal red muscle of rainbow trout (Salmo gairdnerii) have remarkably high activity of branched chain alpha-keto acid dehydrogenase as measured with alpha-keto isocaproic acid as substrate. The activity of the dehydrogenase increases several-fold after preincubation of the mitochondria with an uncouple and oligomycin. The accumulation of the first product, isovaleryl-CoA is strongly stimulated by high concentrations of CoASH. In the crude mitochondrial lysates we have used, an unknown compound accumulates with time, during incubation with alpha-keto isocaproic acid, CoASH and NAD. The compound, probably a CoA derivative, is more hydrophobic than isovaleryl-CoA.
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关键词
rainbow trout
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