Purification and some properties of β-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404

Jun Yoshikawa,Seigo Amachi,Hirofumi Shinoyama,Takaaki Fujii

Journal of Bioscience and Bioengineering（2007）

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摘要

β-Fructofuranosidase I (FFase I) formed by Aureobasidium pullulans DSM 2404 was purified. The enzyme had a molecular weight of about 430 kDa, was not affected by various metal ions and showed high transfructosylating activity. The yield of fructooligosaccharides production using purified FFase I was 62%.

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关键词

β-fructofuranosidase,Aureobasidium pullulans,fructooligosaccharide,transfructosylation

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