Lysyl-tRNA synthetase of Bacillus stearothermophilus molecular cloning and expression of the gene.

T Takita,N Shimizu,T Sukata,S Hashimoto,E Akita,T Yokota,N Esaki,K Soda,K Inouye,B Tonomura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY（2014）

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摘要

The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids, The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.

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关键词

Bacillus stearothermophilus,gene cloning,gene expression,lysyl-tRNA synthetase

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