Quantitation of Bacillus Clausii in Biological Samples by Real-Time Polymerase Chain Reaction

Mario Perotti,Nicasio Mancini,Annalisa Cavallero,Silvia Carletti,Filippo Canducci,Roberto Burioni,Massimo Clementi

Journal of microbiological methods（2006）

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摘要

A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 10(2) CFU/microl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.

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关键词

Bacillus clausii,probiotics,quantitation,real-time PCR

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