Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus
Journal of Lipid Research(2006)
摘要
The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 2'-O-(1,2-diacyl-sn-glycero-3-phospho) -3'-O-(alpha-N-acetyl-glucosaminyl)N-glyceroyl alkylamine [PGL1 (1)] and the novel structure 2'-O-(2-acylalkyldio-1-O-phospho)-3'-O-(alpha-N-acetylglucosaminyl)-N-glyceroyl alkylamine [PGL2 (2)]. PGL2 (2) is the first phosphoglycolipid identified with a 2-acylalkyldio-1-Ophosphate moiety. The fatty acids of the phosphoglycolipids are mainly iso-C-15:0, -C-16:0, and -C-17:0 and anteiso-C-15:0 and -C-17:0. The ratios of PGL2 (2) to PGL1 (1) are significantly altered when grown at different temperatures for three strains, T. thermophilus NTU-077, M. ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimai NTU-063.10 Accordingly, the ratios of iso- to anteiso-branched fatty acids increase when grown at the higher temperature.
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关键词
nuclear magnetic resonance spectroscopy,matrix-assisted laser desorption ionization mass spectroscopy,capillary electrophoresis-mass spectroscopy,tandem mass spectrometry,Thermus oshimai NTU-063,Thermus thermophilus NTU-077,Meiothermus ruber NTU-124,Meiothermus taiwanensis NTU-220
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