MIKROSATELITOVÁ ANALÝZA NA CHROMOZOMU 3 VE TKÁNI KONVENČNÍHO (SVĚTLOBUNĚČNÉHO) RENÁLNÍHO KARCINOMU - MOŽNÝ PROGNOSTICKÝ VÝZNAM

Objective: To evaluate the prognostic value of microsatellite analysis on chromosome 3 in patients with conventional renal cell carcinoma. Methods: A total of 63 patients with localized histologically confirmed renal cell carcinoma were included in a prospective study. The patients underwent nephrectomy or partial resection between 2002 and 2005. We observed the study group for the frequency of loss of heterozygosity (LOH) on chromosome 3 as well as for the relation of loss of heterozygosity to tumour size and grade and/or the course of the disease. Results: Marker D3S1289 was informative in 82.5 % and D3S1560 in 84.1 % of patients. Loss of heterozygosity at locus D3S1289 was shown in 57.1 %, at locus D3S1560 in 55.6 %, and at both loci simultaneously in 42.9 % of cases. During a mean follow-up of 29.5 months, the disease recurred in 7 patients. In each of them, LOH was confirmed in at least one of the loci, in four of them in both loci. Conclusion: Loss of heterozygosity (LOH) at loci D3S1560 and D3S1289 is a common finding in localized conventional renal cell