Identification Of 2-(Cysteinyl)Amido-2-Deoxy-D-Galacturonic Acid Residue From The Sheath Of Leptothrix Cholodnii
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY(2006)
摘要
The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.
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关键词
Leptothrix cholodnii,2-(cysteinyl)amido-2-deoxy-D-galacturonic acid,sheath
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