Effects of combined expression of antifungal barley seed proteins in transgenic wheat on powdery mildew infection
Molecular Breeding(2003)
摘要
Transgenicwheat plants (variety Frisal) constitutively expressing a number of potentialantifungal proteins alone or in combinations were generated and tested forincreased resistance to Blumeria graminis f.sp. tritici(powdery mildew) in a detached leaf infection assay. The most significativerateof protection was obtained with an apoplastic ribosome-inactivation proteinfrombarley seed. Apoplastic Barnase was less efficient and individual plant linesharbouring a barley seed chitinase and β-1,3-glucanase showed linespecificphenotypes from increased resistance to increased susceptibility. Combinationbycrossing of three barley seed proteins did not lead to significant improvementof protection.
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关键词
beta-1,3-glucanase,anti-fungal protein,chitinase,fungal tolerance,ribosome inactivating protein,synergistic effect,transgenic wheat
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