The Ubiquitin C-terminal Hydrolase L-1 (PGP9.5) as a suitable marker to trace and isolate spermatogonia cells from ovine testis

The Ubiquitin C-terminal Hydrolase L-1 (UCH-L1) is usually named as PGP9.5. Earlier study indicated that PGP9.5 has been used to identify type A spermatogonia in some species such as mouse and pig. In the present study, the localization of PGP9.5 was studied on paraffin sections of the ram testis samples in order to understand the correlation between gonocyte migration and spermatogonia location in the seminiferous tubules of ovine testis development. The results also indicated that testes from neonatal ovine had only one type of undifferentiated germ cell gonocyte and PGP9.5 as a spermatogonia cell marker was suitable for isolation and enrichment of ovine Spermatogonial Stem Cells (SSCs). Thus, the finding will be helpful for the future study on ovine SSCs in long-term culture and transplantation.