Genetic modification of Teredinibacter turnerae, an endosymbiont with biotechnological potential.

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY(2010)

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摘要
Teredinibacter turnerae belongs to a group of biotechnologically relevant bacteria. Gene transfer into T. turnerae was achieved by using pPROBE'-gfp[ASV] derived plasmids through conjugative mating with Escherichia coli DH5 alpha pRK2073. Transferred plasmids were stably maintained and T. turnerae could also act as a donor to transfer these mobilizable plasmids. Constructs for both constitutive and IPTG-inducible gene expression were obtained, representing new tools for gene overexpression in T. turnerae. Copyright (C) 2010 S. Karger AG, Basel
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关键词
Teredinibacter turnerae,Cellulolytic/nitrogen-fixing bacteria,Genetic modification,Conjugative transfer
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