Quantitation Of Cytomegalovirus (Cmv) Dna In Leukocytes Of Human Immunodeficiency Virus-Infected Subjects With And Without Cmv Disease By Using Pcr And The Sharp Signal Detection System

We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.