Luciferase activity of the intracellular microcrystal of the firefly squid, Watasenia scintillans.
FEBS Letters(2011)
摘要
The arm light organ of the firefly squid, Watasenia scintillans, emits extremely bright flashes of light, which are caused by a luciferin-luciferase reaction involving ATP, Mg(2+) and molecular oxygen. The molecular mechanism underlying the bioluminescence reaction has remained unresolved, because the luciferase could not be identified or isolated. The arm light organ contains numerous rod-like bodies that are 2-6 μm long and 1-2 μm thick. This paper addresses the characterization of the extracted rod-like body. We found that the rod-like bodies emit the light in vitro by the luciferin-luciferase reaction. Furthermore, by using the X-ray powder diffraction method, we confirmed that the rod-like bodies are well-ordered microcrystals.
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关键词
Bioluminescence,Luciferase,Intracellular microcrystal,Firefly squid,X-ray powder diffraction
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