Identification of the mutations in the prfB gene of Escherichia coli K12, which confer UGA suppressor activity.
JAPANESE JOURNAL OF GENETICS(1990)
摘要
By DNA sequencing and gene dissection, it has been revealed that Su+UGA#11, the mutant prfB of E. coli (Chang et al., 1990) has a double mutation compared with the wild-type LS653: one is a base substitution from T to C at the codon 63 and the other is from G to A at the codon 79. Both mutations cause amino acid substitution, Leu63----Phe63 (L63F) and Asp79----Gly79 (D79G), and are necessary to confer the efficient UGA suppressor activity.
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关键词
escherichia coli
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