Affinity chromatographic procedure for the quantitative recovery of DNA fragments from agarose gels
Analytical Biochemistry(1986)
摘要
A simple and reliable method for the recovery of specific fragments of DNA from agarose gels is presented. The electroelution of the DNA onto the NENSORB cartridge matrix with the subsequent elution of the bound DNA by a methanol (50% vv) wash has been shown to result in the quantitative recovery of the restriction fragment. Of importance is the fact that the DNA purified by this procedure is a viable substrate for further digestion by a second restriction endonuclease. The method does not require either phenol extraction or extensive desalting of the sample.
更多查看译文
关键词
DNA purification,electroelution,restriction fragments,agarose gel electrophoresis,DNA-binding matrix
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要