A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility

Journal of biomolecular NMR, Volume 51, Issue 4, 2011, Pages 449-456.

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Abstract:

An easy to use and robust approach for amino acid type selective isotope labeling in insect cells is presented. It relies on inexpensive commercial media and can be implemented in laboratories without sophisticated infrastructure. In contrast to previous protocols, where either high protein amounts or high incorporation ratios were obtain...More

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