Determining the number of specific proteins in cellular compartments by quantitative microscopy

NATURE PROTOCOLS, pp. 1953.0-1968.0, 2011.

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Abstract:

T his protocol describes a method for determining both the average number and variance of proteins, in the few to tens of copies, in isolated cellular compartments such as organelles and protein complexes. Other currently available protein quantification techniques either provide an average number, but lack information on the variance, or...More

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