Retrospective detection of exposure to organophosphorus anti-cholinesterases: mass spectrometric analysis of phosphylated human butyrylcholinesterase.

A Fidder,A G Hulst,D Noort,R de Ruiter,M J van der Schans,H P Benschop,J P Langenberg

CHEMICAL RESEARCH IN TOXICOLOGY（2002）

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摘要

In this paper a novel and general procedure is presented for detection of organophosphate-inhibited human butyrylcholinesterase (HuBuChE), which is based on electrospray tandem mass spectrometric analysis of phosphylated nonapeptides obtained after pepsin digestion of the enzyme. The utility of this method is exemplified by the positive analysis of serum samples from Japanese victims of the terrorist attack with sarin in the Tokyo subway in 1995.

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关键词

ergonomics,enzyme activity,terrorism,cholinesterase,cholinesterase inhibitors,mass spectrometry,hydrolysis,injury prevention,colorimetry,environmental monitoring,suicide prevention,spectrometry,enzyme assay,protein phosphorylation,human factors,high performance liquid chromatography,occupational safety

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