Single-molecule fluorescence quantification with a photobleached internal standard.
ANALYTICAL CHEMISTRY, no. 24 (2012): 10522-10525
In cellular and molecular biology, fluorophores are employed to aid in tracking and quantifying molecules involved in cellular function. We previously developed a sensitive single-molecule quantification technique to count the number of proteins and the variation of the protein number over the population of individual subcellular organell...More
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