A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections.

•We present a novel indirect immunofluorescent test for anti-HEV Ig detection.•Stained mammalian cells transiently express recombinant ORF2 protein.•The test confirms commercial anti-HEV IgG assays and detects reliably anti-HEV IgA.•The assessment of the signal localization and staining pattern is a crucial factor.•Our protocol is simple and feasible under diagnostic laboratory conditions.