Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation

Danielle L Swaney,Pedro Beltrao,Lea Starita,Ailan Guo,John Rush,Stanley Fields,Nevan J Krogan,Judit Villén

Nature methods（2013）

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摘要

Two methods for identifying protein isoforms that are concurrently phosphorylated and ubiquitylated are applied in yeast to identify phosphorylation sites that regulate ubiquitin proteasome–mediated proteome degradation.

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关键词

Phosphorylation, Proteomics, Ubiquitylation, Proteomic analysis

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