(1)H magnetic resonance spectroscopy of preinvasive and invasive cervical cancer: in vivo-ex vivo profiles and effect of tumor load.

Purpose:, To compare in vivo H-1 magnetic resonance (MR) spectra of preinvasive and invasive cervical lesions with ex vivo magic angle spinning (MAS) spectra of intact biopsies from the same subjects and to establish the effects of tumor load in the tissue sampled on the findings. Materials and Methods: A total of 51 subjects (nine with normal cervix, 10 with cervical intraepithelial neoplasia. [CIN], and 32 With cervical cancer) underwent endovaginal MR at 1.5 T. Single-voxel (3.4 cm(3)) H-1 MR spectra were acquired and voxel tumor load was calculated (tumor volume within voxel as a percentage of voxel volume). Resonances from triglycerides -CH2 and -CH3 and choline-containing compounds (Cho) were correlated with voxel tumor load. Biopsies analyzed by H-1 MAS-MR spectroscopy (MRS) had metabolite level's correlated with tumor load in the sample at histology. Results: In vivo studies detected Cho in normal, CIN, and cancer patients with no significant differences in levels (P 0.93); levels were independent of voxel tumor load. Triglyceride -CH2 and -CH3 signals in-phase with Cho were present in 77% and. 29%, respectively, of cancer subjects (but not in normal women or those with CIN), but did not correlate with voxel tumor load. Ex vivo. cancer biopsies., showed levels of triglycerides -CH2 and -CH3 and of Cho that were significantly greater than in normal or CIN biopsies (P < 0.05); levels were independent of the tumor load in, the sample. The. presence of -CH2, in vivo predicted the specificity of 77.4% and 93.8% respectively, positive (PPV) and negative (NPV) predictive values were 96% and 68.2%; for -CH2 ex vivo, sensitivity was 100%; specificity, 69%; PPV, 82%; and NPV, 100%. Conclusion: Elevated lipid levels are detected by MRS in vivo and ex vivo in cervical cancer and are independent of tumor load in the volume of tissue sampled.