Virus Detection in Bronchoalveolar Lavage Fluid of 122 Children with Severe Pneumonia

Objective To investigate the prevalence of viral pathogen in children with severe pneumonia in Hunan.Method Bronchoalveolar lavage fluid[BALF] were collected from 122 hospitalized children with severe pneumonia in People's Hospital of Hunan province from January 2011 to December 2011.Nested-or reverse transcription Polymerase chain reaction (PCR or RT-PCR)was used to screen Adenovirus (ADV),Human Bocavirus (HBoV),Parainfluenzaviruses1-4 (PIV1-4),Human Respiratory Syneytial virus(RSV),Influenza virus A (IFVA),Influenza virus B (IFVB),Human Rhinovirus(HRV),Human Metapneumovirus (HMPV),human coronaviruses NL63 and HKU1 (HCoV-NL63,HCoV-HKU1).Results Among the 122 bronchoalveolar lavage fluid,viral agents were detected in 60 samples(49.1％),among which ADV (40.98％) was the most common virus,followed by RSV (7.37％) and HBoV (7.37％).Two viruses were detected in 21 individual (35％) samples,of which 20 were dual positive for ADV (40％).Conclusion ADV is the most frequently detected viral etiology of severe pneumonia in children in Hunan during this year.And its Coinfection with other respiratory viruses was common.