Identification of Murine Basophils by Flow Cytometry and Histology

Here, we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4(-) CD19(- )CD49b(+)IgE(+) cells in flow cytometric analysis. When IgE levels are low, one can also use anti-FcERI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining, we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.