Dynamin 2 interacts with connexin 26 to regulate its degradation and function in gap junction formation.

•We identified dynamin 2 (Dyn2) as a Cx26 interactor in yeast and mammalian cells.•Interaction involves the C-terminus of Cx26 and the GTPase effector domain of Dyn2.•Dyn2 inhibition using siRNA or dynasore resulted in reduced Cx26 degradation.•Dyn2 regulates Cx26 endocytosis and ubiquitination.•Our results establish Dyn2 as a Cx26 partner in the regulation of GJIC.