Combined 13 C NMR- and mass-spectrometry for non-invasive monitoring of metabolism

A method for the long-term incubation of cell suspensions at measured dissolved gas concentrations during acquisition of NMR spectra is described: a mass spectrometer probe in the cell suspension provides continuous monitoring of gases. Its usefulness is shown for Trichomonas vaginalis where fermentation products from glucose in 72 μM O2 are different from those under strictly anaerobic conditions.