Molecular detection of Babesia ovis in sheep and ticks using the gene encoding B. ovis surface protein D ( Bo SPD)

•The gene termed Babesia ovis surface protein D (BoSPD) was cloned.•A PCR based on the BoSPD gene sequence was developed.•BoSPD PCR enabled detection of B. ovis in prolonged infection in lambs and in ticks.•BoSPD PCR was used to detect B. ovis in naturally-infected sheep and ticks.