Molecular Profiling of Dendrocalamus asper and D. Hamiltonii Using RAPD, ISSR and SSR Markers - Effects of DNA Extraction Methods on PCR Amplification

Extraction of good quality DNA is the most essential requirement for molecular analysis of any species. Five protocols for DNA extraction were compared to assess their effects on amount of DNA recovered, its purity and efficacy in PCR amplification using RAPD, ISSR and SSR markers in two species of Dendrocalamus asper. and D. hamiltonii. L eaf tissues from in vitro cultures , tissue culture raised plants in green house,and young and mature leaves from field grown plants of the two species were subjected to five DNA extraction methods using the protocols. Significant differences in DNA quantity and quality between samples and protocols were observed. Leaves of tissue culture raised plants growing in the green house gave best DNA yield (5681, 5015 ng/ μl)followed by in vitro shoot cultures (4645, 4379 ng/ μl), and young (2720, 1801 ng/ μl) and mature leaves (1800, 1368 ng/ μl) from field grown plants of D. hamiltonii and D. asper respectively.The concentration of DNA obtained was more for D. hamiltonii samples than the corresponding D. asper tissues .