Cell Adhesion of Human Bone Marrow-derived Mesenchymal Stem (HMS0014) Cells on Titanium Discs with Different Surface Modifications
Journal of oral medicine(2013)
摘要
The modification of surface microtextures on the implant fixture increases direct bone-to-implant contact compatibility to benift primary stability and osseointegration of dental implant therapy. Cell proliferation and differentiation of HMS0014 cells, and mineralisation of the ECM in monolayer cell culture were preliminarily studied. Subsequently, the HMS0014 cells were GBR-engineered to initiate osteogenesis on either titanium (Ti) or Ti alloy discs modified with different surface substrates. Attachment onto the substratum, extension and intercellular contact of the HMS0014 cells under inducing condition were studied with fluorescent immunohistology and conventional scanning electron microscopy (SEM). The present study obtained results as follows: (i) The SEM demonstrated that the spherical-to-polygonal (d= 10-40μm) HMS0014 cells proliferated and differentiated into flat polygonal (30x90-100x200 μm2) cells, showing prominent lamellipodia and dendritic filopodia, to employ cell-to-substrate and intercellular attachments on the Ti disc surface between 60 to 180 minutes of culture. (ii)The fluorescent immunohistochemistry demonstrated the co-expression of F-actin (actin filament, cytoskeleton protein) and CD51 (α V integrin, interfacing specific receptor protein) in the attached HMS0014 cells. (iii) The matured osteoblast-like HMS0014 cells initiated mineralisation on day 1 of culture; distribution of calcification loci in the ECM was prominently observed between days 7 and 14 of culture. We concluded that the present GBR method enhanced HMS0014 cells to initiate contact osteogenesis on Ti and Ti alloy discs subject to different surface modifications.
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