Micro propagation of Carrizo (Citrus carrizo) through mature bud culture.

The influence of the basal medium and different plant growth regulators on micropropagation of nodal explants from mature trees Carrizo cultivars were investigated. Although the basal medium did not affect. several combinations of 6-benzyl amino purine (BAP) and gibberellic acid (GA3) were used to optimise the proliferation phase. The number of shoots was dependent on the BAP and GA3 concentrations and the best results were obtained with BAP 1 mg /L + GA3 1 mg/L. Explants length was shorter with the higher BAP concentrations and, shoot length was greater with increasing levels of GA3. The best results for productivity (number of shoots 7.9 and the average shoot length 3.0 cm) were obtained with 1 mg/L BAP and 1 mg/L GA3, although explants with narrow leaves were observed. The presence of BAP and GA3 in the proliferation medium was essential for the explants multiplication but BAP had a greater influence in shoot multiplication and GA3­ influence in elongation of shoots.