In vivo imaging of the absorption and scattering properties of exposed rat brain by using multi-spectral diffuse reflectance images

Determining optical properties of brain tissue is important for application of light in clinical diagnosis, surgery, and therapeutic procedure in brain disorders. Diffuse reflectance spectroscopy (DRS) is one of most promising technique for evaluating cortical hemodynamics [1] and assessment of tissue viability [2]. In this study, we investigate a method to estimate the spectral images of reduced scattering coefficients μs’(λ) and the absorption coefficients μa (λ) of in vivo exposed brain tissues in the range from visible to near-infrared wavelength (500-760 nm) based on DRS using a multi-spectral imaging system.