Spr Techniques For Characterization Of Adsorption Layers And Sensitive Measurements Of Biomolecular Interactions

A sensitive biosensor based on photodiode-array registration of the surface plasmon resonance (SPR) was developed. The detection limit of about 3 pg/mm(2) far an adsorption layer was reached, This value is in agreement with the estimates mode for shot noise limited operation. The sensor was used for characterization of protein layers as well as for the study of the binding kinetics of small cap-molecules to a protein immobilized onto the gold film.