A Highly Active Bile Salt Hydrolase from Enterococcus faecalis Shows Positive Cooperative Kinetics

•The cloning, expression and purification of BSH from Enterococcus faecalis were elucidated.•Biochemical and biophysical studies of EfBSH were conducted.•The enzyme showed high activity and positive cooperativity.•Pen V exerted a modulating effect on BSH activity towards conjugated bile acids.