Hydrogen Peroxide Modulates Electrophysiological Characteristics Of Left Atrial Myocytes

Background: Oxidative stress plays an important role in the pathophysiology of atrial fibrillation (AF). The hydrogen peroxide (H2O2) mainly underlies the cellular oxidative stress and free radicals. Left atrium (LA) is the most important AF substrate. However, the effects of H2O2 on the action potential (AP) and ionic currents in LA myocytes have not been fully elucidated.Methods: The whole-cell patch clamp was used to investigate the APs and ionic currents of L-type calcium current (Ica-L), transient outward currents (I-to), ultra-rapid delayed rectifier potassium current (I-kur), delayed rectifier Kur,, potassium currents (I-K), inward rectifier potassium current (I-K1), and sodium-calcium exchanger (NCX) before and after H2O2(100 mu M) in isolated rabbit LA myocytes.Results: H2O2 (100 mu M) shortened by 50% (from 40 +/- 7 to 21 +/- 5 ms) and 90% the AP duration (from 95 +/- 12 to 74 +/- 11 ms) in LA myocytes (n = 9), but did not change the resting membrane potentials. The H2O2 (100 mu M) decreased I-to, but increased I-Kur and I-K. H2O2 (100 mu M) also reduced the 'Ca-L and the reverse mode NCX. However, H2O2 (100 mu M) did not change /10..Conclusions: H2O2 directly modulated the AP morphology and ionic currents in LA myocytes, which may contribute to the genesis of AF in oxidative stress.