Effects of re-stripping on the seminal characteristics of pacu (Piaractus mesopotamicus) during the breeding season

•Milt volume increases over time, but DNA quality and sperm motility decrease.•Morphological abnormalities in sperm were correlated with sperm DNA damage.•Re-stripping was possible for 100% of the spermiating males at the beginning of the season.•Re-stripping might increase the milt volume and improve semen characteristics.•Good-quality semen characteristics are reduced at the end of the spawning season.