Data analysis considerations in probing single quantum dot fluorescence intermittency
Proceedings of SPIE(2005)
摘要
The fluorescence intermittency of single, bare, CdSe/ZnS quantum dots was probed using single molecule confocal microscopy and found to demonstrate power law kinetics. Various threshold values and line fitting parameters are employed in the data analysis and their effects on the extracted power law exponents, m(off) and m(on), are presented. The threshold is found to be critical in determining m(off) while having no significant effect on m(on). The mean plus 2 sigma threshold, calculated from the background noise in the measurement, results in a more negative m(off) slope in comparison to the mean plus 3 sigma or mean plus 4 sigma thresholds. This is likely due to the mean plus 2 sigma threshold lying within the background noise outliers which mimic short on events. In contrast, the mean plus 4 sigma threshold is above 99.99% of the background noise while adequately below the fluorescence signal. Additionally, it is found that fitting only the ten most probable data points rather than all the data points in the log-log probability density graphs results in no significant change in m(off) and m(on).
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关键词
quantum dots,fluorescence intermittency,blinking,threshold analysis,single molecule microscopy
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