SELECTIVE MONITORING OF STERN-VOLMER QUENCHING OF CHIRAL MOLECULES VIA FLUORESCENCE DETECTED CIRCULAR-DICHROISM
Analytical Letters(1987)
摘要
The use of fluorescence detected circular dichroism (FDCD) for monitoring the quenching of chiral molecules is described. This technique combines the specificity for chiral centers of circular dichroism (CD), the sensitivity and selectivity of the fluorescence measurement with the additional chemical information provided by fluorescence quenching. Spectra are acquired using a multidimensional FDCD spectrometer which obtains a FDCD matrix of spectral data as a function of multiple excitation wavelengths (CD information) and multiple fluorescence emission wavelengths. This combination of FDCD and fluorescence quenching produces a very selective monitor for chiral fluorophores.
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关键词
fluorescence quenching,circular dichroism
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