The use of metabolomics to monitor simultaneous changes in metabolic variables following supramaximal low volume high intensity exercise
Metabolomics(2015)
摘要
High intensity exercise (HIE) stimulates greater physiological remodeling when compared to workload matched low-moderate intensity exercise. This study utilized an untargeted metabolomics approach to examine the metabolic perturbations that occur following two workload matched supramaximal low volume HIE trials. In a randomized order, 7 untrained males completed two exercise protocols separated by 1 week; (1) HIE 150 % : 30 × 20 s cycling at 150 % VO 2peak , 40 s passive rest; (2) HIE 300 % : 30 × 10 s cycling at 300 % VO 2peak , 50 s passive rest. Total exercise duration was 30 min for both trials. Blood samples were taken at rest, during and immediately following exercise and at 60 min post exercise. Gas chromatography-mass spectrometry analysis of plasma identified 43 known metabolites of which 3 demonstrated significant fold changes (HIE 300 % compared to the HIE 150 % value) during exercise, 14 post exercise and 23 at the end of the recovery period. Significant changes in plasma metabolites relating to lipid metabolism [fatty acids: dodecanoate (p = 0.042), hexadecanoate (p = 0.001), octadecanoate (p = 0.001)], total cholesterol (p = 0.001), and glycolysis [lactate (p = 0.018)] were observed following exercise and during the recovery period. The HIE 300 % protocol elicited greater metabolic changes relating to lipid metabolism and glycolysis when compared to HIE 150 % protocol. These changes were more pronounced throughout the recovery period rather than during the exercise bout itself. Data from the current study demonstrate the use of metabolomics to monitor intensity-dependent changes in multiple metabolic pathways following exercise. The small sample size indicates a need for further studies in a larger sample cohort to validate these findings.
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关键词
Supramaximal exercise,Metabolomics,Metabolism,Low volume
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