Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC–MS/MS and in silico analysis

•We developed an integrated approach to screen DPP-IV inhibitory peptides.•5.9%~20.9% peptides of deer skin hydrolysates have a Pro at penultimate position.•Methionine oxidation and length of peptide affected their DPP-IV inhibitory activity.•The integrated approach is efficient to identify bioactive peptides.