Rhamnolipids functionalized AgNPs-induced oxidative stress and modulation of toxicity pathway genes in cultured MCF-7 cells

•Biosurfactant rhamnolipids were extracted from P. aeruginosa culture extract.•Non-toxic concentration (0.005%) of rhamnolipids was used for synthesis of Rh-AgNPs.•Rh-AgNPs exhibited more cytotoxicity to MCF-7 cells than normal PBMN cells.•Rh-AgNPs caused ROS generation, membrane damage and apoptosis in MCF-7 cells.•Oxidative stress pathway genes are implicated in Rh-AgNPs induced MCF-7 cells death.