Immunoproteomic and bioinformatic approaches to identify secreted Leishmania amazonensis, L. braziliensis, and L. infantum proteins with specific reactivity using canine serum.

•Immunoproteomic approaches was used to identify reactive proteins in Leishmania spp.•Twenty-eight non-redundant immunogenic proteins were identified.•Bioinformatic analyses highlighted six putative secreted proteins with specific reactivity.•These proteins may be used as targets for diagnosis of Leishmania infection.