Procedures and applications of long-term intravital microscopy.

•Long-term IVM enables repetitive imaging over days to months within the same animal.•Procedures, Pros & Cons, and Tips & Tricks of different imaging windows are described.•It is addressed how a position of interest can be retraced over multiple days.•Different tools to monitor cell migration and proliferation are explained.•Long-term two-photon IVM studies focusing on proliferation and migration are reviewed.