Structure and diversity of human dendritic spines evidenced by a new three-dimensional reconstruction procedure for Golgi staining and light microscopy.
Journal of Neuroscience Methods(2018)
摘要
•Human dendritic spines were studied from formalin fixed tissue stored for long time.•The “single-section” Golgi method provided suitable data for 3D reconstruction.•Procedure is relatively easy, requires minimal equipment and computer software.•A new algorithm provides detailed images with high quality using light microscopy.•Human spines show a continuum of shapes and sizes at different visualizing angles.
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关键词
Image enhancement and binarization,Image segmentation,Human brain,Spine density,Spine shape,3D reconstruction
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