Preparation of polysomal fractions from mouse brain synaptoneurosomes and analysis of polysomal-bound mRNAs.

Bozena Kuzniewska,Magdalena Chojnacka,Jacek Milek,Magdalena Dziembowska

Journal of Neuroscience Methods（2018）

引用 16|浏览4

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摘要

•A step-by-step reliable protocol to prepare synaptoneurosomes from rodent brain.•Polyribosome fractionation from synaptoneurosomes.•Extraction of proteins and RNA from sucrose gradient fractions.

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关键词

Synaptoneurosomes,Polyribosome fractionation,RNA,Protein

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