Analysis of adaptive mutations selected during the consecutive passages of hepatitis E virus produced from an infectious cDNA clone.

Shigeo Nagashima,Tominari Kobayashi,Toshinori Tanaka,Tanggis,Suljid Jirintai,Masaharu Takahashi,Tsutomu Nishizawa,Hiroaki Okamoto

Virus Research（2016）

引用 7|浏览4

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摘要

•A cloned genotype 3 HEV (pJE03-1760F/wt) was passaged 10 times in A549 cells.•The Passage 10 virus had two non-synonymous and four synonymous mutations.•Full-length cDNA clones with a single, two, or all six mutations were constructed.•HEV with all six mutations produced 2 logs more virions than did starting virus.•Both non-synonymous and synonymous HEV mutations enhanced virus production.

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关键词

Adaptation,Cell culture,Hepatitis E virus,Infectious cDNA clone,Reverse genetics system,Synonymous mutation

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